2510: Simplified and rapid method for extraction of ergosterol from natural samples and detection with quantitative and semi-quantitative methods using thin-layer chromatography
(2004) Simplified and rapid method for extraction of ergosterol from natural samples and detection with quantitative and semi-quantitative methods using thin-layer chromatography. Journal of Chromatography A 1026:301 - 304.*
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Summary
A new and simplified method for extraction of ergosterol (ergoste-5,7,22-trien-3-beta-ol) from fungi in soil and litter was developed using pre-soaking extraction and paraffin oil for recovery. Recoveries of ergosterol were in the range of 94 - 100% depending on the solvent to oil ratio. Extraction efficiencies equal to heat-assisted extraction treatments were obtained with pre-soaked extraction. Ergosterol was detected with thin-layer chromatography (TLC) using fluorodensitometry with a quantification limit of 8 ng. Using visual evaluation of images of TLC plates photographed in UV-light the quantification limit was 16 ng.
| Document Language: | English |
|---|---|
| Keywords: | Ergosterol; TLC; Extraction; Fungi;Litter; Soil |
| Subject Areas: | Soil > Soil quality > Soil biology |
| Research affiliation: | Denmark > DARCOF II (2000-2005) > I. 3 (BIOMOD) Interaction between nitrogen dynamics, crop production and biodiversity |
| Funding Part: | 25-75% |
| Orgprints ID Number: | 2510 |
| Contact: | Axelsen, Senior Scientist Jørgen |
| Deposited On: | 16 April 2004 |
| EPrint Type: | Journal paper |
| Published?: | Published |
| Peer Review Status: | Peer-reviewed and accepted |
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