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Simplified and rapid method for extraction of ergosterol from natural samples and detection with quantitative and semi-quantitative methods using thin-layer chromatography

Larsen, Cand.scient Thomas; Ravn, Senior scientist Helle and Axelsen, Senior Scientist Jørgen (2004) Simplified and rapid method for extraction of ergosterol from natural samples and detection with quantitative and semi-quantitative methods using thin-layer chromatography. Journal of Chromatography A, 1026, 301 - 304.

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Summary

A new and simplified method for extraction of ergosterol (ergoste-5,7,22-trien-3-beta-ol) from fungi in soil and litter was developed using pre-soaking extraction and paraffin oil for recovery. Recoveries of ergosterol were in the range of 94 - 100% depending on the solvent to oil ratio. Extraction efficiencies equal to heat-assisted extraction treatments were obtained with pre-soaked extraction. Ergosterol was detected with thin-layer chromatography (TLC) using fluorodensitometry with a quantification limit of 8 ng. Using visual evaluation of images of TLC plates photographed in UV-light the quantification limit was 16 ng.


EPrint Type:Journal paper
Keywords:Ergosterol; TLC; Extraction; Fungi;Litter; Soil
Subjects: Soil > Soil quality > Soil biology
Research affiliation: Denmark > DARCOF II (2000-2005) > I. 3 (BIOMOD) Interaction between nitrogen dynamics, crop production and biodiversity
Deposited By: Axelsen, Senior Scientist Jørgen
ID Code:2510
Deposited On:16 Apr 2004
Last Modified:12 Apr 2010 07:29
Document Language:English
Status:Published
Refereed:Peer-reviewed and accepted

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