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High-resolution analysis of a QTL for resistance to Stagonospora nodorum glume blotch in wheat reveals presence of two distinct resistance loci in the target interval

Shatalina, Margarita; Messmer, Monika; Feuillet, Catherine; Mascher, Fabio; Paux, Etienne; Choulet, Frédéric; Wicker, Thomas and Keller, Beat (2013) High-resolution analysis of a QTL for resistance to Stagonospora nodorum glume blotch in wheat reveals presence of two distinct resistance loci in the target interval. Theoretical and Applied Genetics (Article published online: 04 december 2013), 127 (3), pp. 573-586.

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Online at: http://link.springer.com/article/10.1007%2Fs00122-013-2240-4

Summary

Stagonospora nodorum glume blotch (SNG), caused by the necrotrophic fungus Stagonospora nodorum, is one of the economically important diseases of bread wheat (Triticum aestivum L.). Resistance to SNG is known to be quantitative and previous studies of a recombinant inbred line (RIL) population identified a major quantitative trait locus (QTL) for resistance to SNG on the short arm of chromosome 3B. To localize this QTL (QSng.sfr-3BS) with high resolution, we constructed a genetic map for the QTL target region using information from sequenced flow-sorted chromosomes 3B of the two parental cultivars ‘Arina’ and ‘Forno’, the physical map of chromosome 3B of cultivar ‘Chinese Spring’ and BAC-clone sequences. The mapping population of near-isogenic lines (NIL) was evaluated for SNG resistance in field infection tests. NILs segregated for disease resistance as well as for plant height; additionally, we observed a high environmental influence on the trait. Our analysis detected a strong negative correlation of SNG resistance and plant height. Further analysis of the target region identified two linked loci associated with SNG resistance. One of them was also associated with plant height, revealing an effect of QSng.sfr-3BS on plant height that was hidden in the RIL population. This result demonstrates an unexpectedly high genetic complexity of resistance controlled by QSng.sfr-3BS and shows the importance of the study of QTL in mendelized form in NILs.


EPrint Type:Journal paper
Keywords:Pflanzenzüchtung, Pflanzenbauwissenschaften, Stagonospora nodorum glume blotch (SNG), Weizen, wheat, Pflanzengesundheit, resistance
Subjects: Crop husbandry > Production systems > Cereals, pulses and oilseeds
Crop husbandry > Breeding, genetics and propagation
Crop husbandry > Crop health, quality, protection
Research affiliation: Switzerland > FiBL - Research Institute of Organic Agriculture Switzerland > Soil Sciences
Switzerland > Agroscope > ACW - Research Centre Wädenswil
Switzerland > Zürich University
France > INRA - Institut National de la Recherche Agronomique
ISSN:0040-5752, online: 1432-2242
DOI:10.1007/s00122-013-2240-4
Deposited By: Messmer, Dr. Monika
ID Code:25038
Deposited On:21 Jan 2014 21:51
Last Modified:16 Jun 2014 12:26
Document Language:English
Status:Published
Refereed:Peer-reviewed and accepted

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