home    about    browse    search    latest    help 
Login | Create Account

Regulation of cytochrome P450 mRNA expression in primary porcine hepatocytes by selected secondary plant metabolites from chicory (Cichorium intybus L.)

Rasmussen, Martin Krøyer; Klausen, Christina Lindgaard and Ekstrand, Bo (2013) Regulation of cytochrome P450 mRNA expression in primary porcine hepatocytes by selected secondary plant metabolites from chicory (Cichorium intybus L.). Food Chemistry, 146 (1), pp. 255-263.

[thumbnail of 24791.pdf] PDF - English
Limited to [Depositor and staff only]

1MB


Summary

Chicory (Cichorium intybus) has been shown to induce enzymes of pharmacokinetic relevance (cytochrome P450; CYP). The aim of this study was to investigate the effects of selected secondary plant metabolites with a global extract of chicory root, on the expression of hepatic CYP mRNA (1A2, 2A19, 2C33, 2D25, 2E1 and 3A29), using primary porcine hepatocytes. Of the tested secondary plant metabolites, artemisinin, scoparone, lactucin and esculetin all induced increased expression of specific CYPs, while esculin showed no effect. In contrast, a global extract of chicory root decreased the expression of CYP1A2, 2C33, 2D25 and 3A29 at high concentrations. The results suggest that purified secondary metabolites from chicory affect CYP expression and thereby might affect detoxification in general, and that global extracts of plants can have effects different from individual components.


EPrint Type:Journal paper
Keywords:bioactive compounds, primary hepatocytes, boar taint, liver metabolism, detoxification, gene regulation, cryopreservation
Subjects: Animal husbandry > Feeding and growth
Crop husbandry > Breeding, genetics and propagation
Research affiliation: Denmark > Organic RDD 1 > NO-CAST
DOI:10.1016/j.foodchem.2013.09.068
Deposited By: Madsen, Academic employee Mette Graves
ID Code:24791
Deposited On:04 Dec 2013 13:30
Last Modified:09 Apr 2015 09:55
Document Language:English
Status:Published
Refereed:Peer-reviewed and accepted

Repository Staff Only: item control page

Downloads

Downloads per month over past year

View more statistics