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In vivo effect of dried chicory root (Cichorium intybus L.) on xenobiotica metabolising cytochrome P450 enzymes in porcine liver

Rasmussen, Martin Krøyer; Zamaratskaia, Galia and Ekstrand, Bo (2011) In vivo effect of dried chicory root (Cichorium intybus L.) on xenobiotica metabolising cytochrome P450 enzymes in porcine liver. Toxicology Letters, 200, pp. 88-91.

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Summary

Cytochrome P450 (CYP) enzymes are widely studied for their involvement in metabolism of drugs and endogenous compounds. In porcine liver, CYP1A2,2Aand 2E1 are important for the metabolism of skatole.Feeding chicory roots to pigs is known to decrease the skatole concentration in plasma and fat. In the present study we investigated the effect of chicory on CYP mRNA and protein expression, as well as their activity. Male pigs were feed dried chicory root for 16 days before liver samples were collected. By the use of RT-PCR and Western blotting we showed that the mRNA and protein expression of CYP1A2 and 2A were increased in chicory fed pigs. The mRNA expression of CYP2E1 was increased, while there was no effect on protein expression. Activity of CYP1A2 and 2A were increased in chicory feed pigs; this was not the case for CYP2E1 activity. In conclusion; oral administration of chicory root for 16 days to pigs increased the mRNA expression of CYP1A2, 2A and 2E1; and the protein expression of CYP1A2 and 2A. The activities of CYP1A2 and 2A were increased.


EPrint Type:Journal paper
Keywords:Chicory root, porcine liver, skatole
Subjects: Animal husbandry > Feeding and growth
Research affiliation: Denmark > Organic RDD 1 > NO-CAST
DOI:10.1016/j.toxlet.2010.10.018
Deposited By: Madsen, Academic employee Mette Graves
ID Code:22087
Deposited On:02 Jan 2013 08:17
Last Modified:09 Apr 2015 10:18
Document Language:English
Status:Published
Refereed:Peer-reviewed and accepted

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