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Characterization of microsatellite primers for Bembidion lampros through 454-sequencing of the genome

Marchi, C.; Andersen, L.W.; Panitz, F; Holm, L.-E. and Loeschcke, V. (2012) Characterization of microsatellite primers for Bembidion lampros through 454-sequencing of the genome. Molecular Ecology Resources, , - . [Submitted]

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Summary

454-sequencing of the genome was used to isolate and characterize nuclear 22 microsatellite sequences from the ground beetle Bembidion lampros (Carabidae). A 23 total of 4502 contigs containing di- to penta-nucleotide microsatellite repeats were 24 found (4211 perfect and 289 non-perfect repeats). Primers were developed and tested 25 for 34 microsatellite sequences. Of these 15 were tested on 1140 individual samples 26 amplifying on average 89% of samples and they did not deviate significantly from 27 Hardy-Weinberg and linkage equilibrium. These microsatellite sequences will be useful 28 in studying the effect of agricultural management techniques and various environmental 29 factors on a beneficial agricultural species.


EPrint Type:Journal paper
Subjects:"Organics" in general
Research affiliation: Denmark > DARCOF III (2005-2010) > REFUGIA - The role of Organic Farms as refugia for biodiversity
Deposited By: Hansen, Grethe
ID Code:21072
Deposited On:17 Jul 2012 06:46
Last Modified:17 Jul 2012 06:46
Document Language:English
Status:Submitted
Refereed:Peer-reviewed and accepted

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