Søltoft, Malene; Eriksen, M.R.; Träger, A.W.B.; Nielsen, J.; Laursen, Kristian Holst; Husted, Søren; Halekoh, Ulrich and Knuthsen, Pia (2010) Comparison of Polyacetylene Content in Organically and Conventionally Grown Carrots Using a Fast Ultrasonic Liquid Extraction Method. J Agric Food Chem , 58, pp. 7673-7679.
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A rapid and sensitive analytical method for quantification of polyacetylenes in carrot roots was developed. The traditional extraction method (stirring) was compared to a new ultrasonic liquid processor (ULP)-based methodology using high-performance liquid chromatography−ultraviolet (HPLC−UV) and mass spectrometry (MS) for identification and quantification of three polyacetylenes. ULP was superior because a significant reduction in extraction time and improved extraction efficiencies were obtained. After optimization, the ULP method showed good selectivity, precision [relative standard deviations (RSDs) of 2.3−3.6%], and recovery (93% of falcarindiol) of the polyacetylenes. The applicability of the method was documented by comparative analyses of carrots grown organically or conventionally in a 2 year field trial study. The average concentrations of falcarindiol, falcarindiol-3-acetate, and falcarinol in year 1 were 222, 30, and 94 μg of falcarindiol equiv/g of dry weight, respectively, and 3−15% lower in year 2. The concentrations were not significantly influenced by the growth system, but a significant year−year variation was observed for falcarindiol-3-acetate.
|EPrint Type:||Journal paper|
|Research affiliation:|| Denmark > DARCOF III (2005-2010) > ORGTRACE - Organic food and health|
Denmark > DTU - Technical University of Denmark
Denmark > AU - Aarhus University > AU, DJF - Faculty of Agricultural Sciences
Denmark > KU - University of Copenhagen > KU-LIFE - Faculty of Life Sciences
|Deposited By:||Holst Laursen, Assis Prof Kristian|
|Deposited On:||07 Jul 2011 09:48|
|Last Modified:||07 Jul 2011 09:48|
|Refereed:||Peer-reviewed and accepted|
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