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The role of atomic spectrometry in plant science

Husted, Søren; Persson, D.P.; Laursen, K.H.; Hansen, T.H.; Pedas, P.; Schiller, M.; Hegelund, J.N. and Schjørring, J.K. (2011) The role of atomic spectrometry in plant science. Journal of Analytical Atomic Spectrometry, 26, pp. 52-79.

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Summary

Inductively coupled plasma-mass spectrometry (ICP-MS) is the state-of-the-art technique for multi-elemental analysis of plant tissue. It provides a powerful tool in functional genomics, linking altered elemental profiles of mutants with gene expression and function. In addition, with its unmatched sensitivity, ICP-MS enables characterization of the substrate specificity and regulation of membrane transport proteins. Digestion of plant tissue has traditionally represented a bottleneck due to the low capacity of commercially available equipment. However, recent developments in micro-scaled digestion, combined with semi-quantitative analysis and chemometrics, have enabled high-throughput multi-elemental profiling and multivariate classification of large sample sets, thereby supporting a range of new applications in molecular breeding, quality assessment and authenticity testing of plants. Novel hyphenated techniques based on liquid chromatography and ICP-MS (LC-ICP-MS) have significantly improved the understanding of elemental species and their importance for e.g. the bioactivity of metals in plants. Development of procedures for sample pre-treatment, extraction and multi-dimensional separation now allows characterization of important metallo-biomolecules in plants, such as the coordination complexes of phytochelatins, metallothioneins, nicotianamine and inositol phosphates. These are key ligands involved in ion homeostasis, translocation and long-term storage of elements. Much emphasis has also been given to studies of covalently bound Se and As species, primarily due to their impact on human health. LC-ICP-MS has extensively been complemented by molecular mass spectrometry for structural information of biologically relevant species. This review covers the most recent developments in multi-elemental analysis (Part A) and speciation analysis (Part B) in plant science. A number of relevant cases are presented in order to demonstrate how the analytical developments have unravelled the functional roles of elements in plants science. These cases show that ICP-MS is an essential technology in plant metallomic platforms.


EPrint Type:Journal paper
Subjects: Food systems
Knowledge management
Research affiliation: Denmark > DARCOF III (2005-2010) > ORGTRACE - Organic food and health
Denmark > KU-LIFE - Faculty of Life Sciences
DOI:10.1039/C0JA00058B
Deposited By: Holst Laursen, PhD student Kristian
ID Code:19089
Deposited On:01 Jul 2011 14:10
Last Modified:01 Jul 2011 14:10
Document Language:English
Status:Published
Refereed:Peer-reviewed and accepted

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