Kapolna, Emese; Hillestrøm, Peter; Laursen, Kristian; Husted, Søren and Larsen, Erik H. (2009) Effect of foliar application of selenium on its uptake and speciation in carrot. Food Chemistry, 115, pp. 1357-1363.
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Carrot (Daucus carota) shoots were enriched by selenium using foliar application. Solutions of sodium selenite
or sodium selenate at 10 and 100 lg Se ml�1, were sprayed on the carrot leaves and the selenium content and uptake rate of selenium were estimated by ICP–MS analysis. Anion and cation exchange HPLC were tailored to and applied for the separation of selenium species in proteolytic extracts of the biological tissues using detection by ICP–MS or ESI–MS/MS. Foliar application of solutions of selenite or selenate at 100 lg Se ml�1 resulted in a selenium concentration of up to 2 lg Se g�1 (dry mass) in the carrot root whereas the selenium concentration in the controls was below the limit of detection at 0.045 lg Se g�1 (dry mass). Selenate-enriched carrot leaves accumulated as much as 80 lg Se g�1 (dry mass), while the selenite-enriched leaves contained approximately 50 lg Se g�1 (dry mass). The speciation analyses showed that inorganic selenium was present in both roots and leaves. The predominant metabolised organic forms of selenium in the roots were selenomethionine and c-glutamyl-selenomethyl-
selenocysteine, regardless of which of the inorganic species were used for foliar application. Only selenomethionine was detected in the carrot leaves. The identity of selenomethionine contained in carrot roots and leaves was successfully confirmed by HPLC–ESI–MS/MS.
|EPrint Type:||Journal paper|
|Keywords:||Carrot Foliar application c-glu-MeSeCys SeMet Selenium speciation HPLC–ICP–MS HPLC–ESI–MS/MS|
|Subjects:|| Farming Systems|
|Research affiliation:|| Denmark > DARCOF III (2005-2010) > ORGTRACE - Organic food and health|
Denmark > DTU, DFVF - Danish Institute for Food and Veterinary Research
|Deposited By:||Larsen, Prof Erik H.|
|Deposited On:||07 Jul 2011 12:31|
|Last Modified:||21 Jul 2011 12:28|
|Refereed:||Peer-reviewed and accepted|
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