Jensen, Annette Nygaard and Nielsen, Eva Møller (2003) Campylobacter species distribution in outdoor pigs: Oral presentation O44. In: Leontides, Leonidas (Ed.) Proceedings. SAFEPORK 5th International Symposium on the Epidemiology and Control of Foodborne Pathogens in Pork., pp. 134-136.
A colony blot hybridization method with a digoxigenin-(DIG)labelled nucleotide probe was developed to enable Campylobacter jejuni species-specific identification of colonies present in minority. C. jejuni is the dominant cause of human cases of Campylobacteriosis. In contrast, C. coli is normally dominant in conventional pigs but it can be speculated that outdoor pigs host more C. jejuni due to a closer contact to the environment and wild-life where C. jejuni is pre-dominant. Since C. jejuni is considered more virulent to humans a potential shift towards C. jejuni in pigs may infer an importance to food safety. Individual pigs are often colonized with several Campylobacter strains. However, with conventional culturing techniques only few colonies are typically identified to the species level and thus C. jejuni will only rarely be isolated if present in minority. Using the hybridization method it was possible to identify C. jejuni colonies in 4 out of 20 faecal samples from finisher pigs.
|EPrint Type:||Conference paper, poster, etc.|
|Type of presentation:||Paper|
|Keywords:||organic, pig, campylobacter jejuni, Campylobacter coli, colony hybridization, DIG nucleotide probe,|
|Subjects:|| Food systems > Food security, food quality and human health|
Animal husbandry > Production systems > Pigs
|Research affiliation:|| Denmark > DARCOF II (2000-2005) > II.10 (SaCaFree) Bacterial infection risk associated with outdoor organic pig production ...|
Denmark > SOAR - Research School for Organic Agriculture and Food Systems
|Deposited By:||Jensen, Ms Annette Nygaard|
|Deposited On:||27 Oct 2003|
|Last Modified:||12 Apr 2010 07:28|
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