Proteomics in the backyard: 2DE based comparison of vegetables grown at conventional farming conditions and organic farming conditions

Summary

We used 2D electrophoresis and mass spectrometry to compare two organic farming systems with conventional farming of cabbage and carrot. For both vegetable proteomes we observed differential responses that depended on farming conditions. We detected significant protein abundance changes in a series of metabolic processes including glycolysis and protein synthesis. This study provides a framework for detailed molecular analysis of vegetables in large scale field studies and the influence of environmental conditions on plant growth.