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Ovules Culture and Plant Formation of Hybrid Progeny of Seedless Grape

Guimei, Qi and Hanfeng, Ding (2002) Ovules Culture and Plant Formation of Hybrid Progeny of Seedless Grape. [Embryokulturen und Regeneration bei Kreuzungen samenloser Reben.] In: Boos, Markus (Ed.) Ecofruit - 10th International Conference on Cultivation Technique and Phytopathological Problems in Organic Fruit-Growing and Viticulture. Proceedings to the Conference from 4th to 7th Feb. 2002 at Weinsberg/Germany, pp. 226-230.

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Summary

Ovules of seedless grapes (Vitis spp.) fertilized by controlled pollination increased in size during berry development. Hybrid bunches picked at 30,35,40,45,50 and 55 days after pollination. Ovules were dissected and then cultured in media for 60 to 70 days. Developing ovules were placed in germinative medium. After embryos germinated for 10 days. They were transferred in plant medium to promote them formed normal plants. The result indicated that the most appropriatest medium was Nitsch with GA 30.2m VI, IAA 1.5mgll, ZT1.0 mg/l. The suitable sampling period was after pollination 35 to 50 days. The germinate and the plant rate of 'perlette x' Flame seedless' were the highest in the five crosses, which were 70.2% and 24.8%.
Chemical name used: Gibberellin (GA3), 3-lnddylacticacid (IAA), 6-Benzylaminopurine (6BA), Trans-zeatin (ZT).


EPrint Type:Conference paper, poster, etc.
Type of presentation:Paper
Keywords:ovule culture, embryo germination, grape
Subjects: Crop husbandry > Crop health, quality, protection
Crop husbandry > Production systems > Fruit and berries
Research affiliation: International Conferences > 2002: Ecofruit
Related Links:http://www.ecofruit.net
Deposited By: Tagung, Ecofruit
ID Code:14636
Deposited On:30 Oct 2008
Last Modified:12 Apr 2010 07:38
Document Language:English
Status:Published
Refereed:Peer-reviewed and accepted

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