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Phytic acid degradation by phytase – as viewed by 31P NMR and multivariate curve resolution

Nielsen, Merete Møller; Viereck, Nanna and Engelsen, Søren Balling (2007) Phytic acid degradation by phytase – as viewed by 31P NMR and multivariate curve resolution. In: Farhat, I.; Belton, P.S. and Webb, G.A. (Eds.) Magnetic Resonance in Food Science: From molecules to Man. The Royal Society of Chemistry, pp. 214-222.

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Summary

The 31P NMR method is a most direct and useful method to describe the degradation of phytic acid to lower inositol phosphates by the action of the enzyme phytase. The use of chemometric and CARS visualizes and helps in the interpretation of the results. By means of LatentiX it has been possible to visualize the time-dependent hydrolysis of phytic acid and by PCA the complexity of the phytic acid is shown in the score plots. By modeling the spectra in CARS it is possible to identify and quantify each of the inositol phosphates.


EPrint Type:Book chapter
Subjects: Food systems > Food security, food quality and human health
Research affiliation: Denmark > KU-LIFE - Faculty of Life Sciences
Denmark > SOAR - Research School for Organic Agriculture and Food Systems
Deposited By: Nielsen, Merete Højrup Møller
ID Code:11170
Deposited On:22 Aug 2007
Last Modified:12 Apr 2010 07:35
Document Language:English
Status:Published
Refereed:Not peer-reviewed

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