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RAPD-PCR amplification of DNA from virgin olive oil

Perri, Dr Enzo; Muzzalupo, PhD Innocenzo and Sirianni, Ms Raffaella (2002) RAPD-PCR amplification of DNA from virgin olive oil. In: Vitagliano, C. and Martelli, G. P. (Eds.) Acta Horticulturae, 586, pp. 583-586.

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Summary

The characterization of the genetic identity of DNA recoverable from olive oil could facilitate the identification of the source cultivar. This requires that intact DNA or large fragments suitable for amplification by polymerase chain reaction (PCR) should be recovered from olive oil. This paper presents the preliminary results of an experiment evaluating the extent to which DNA recoverable from the veiled virgin olive (cv. Carolea) oil would allow PCR amplification useful for random amplified polymorphic DNA (RAPD) fingerprinting. Only residual DNA from olive oil sediments obtained by protease treatment was useful in RAPD-PCR amplification.


EPrint Type:Conference paper, poster, etc.
Type of presentation:Paper
Keywords:DNA; olive oil; olives; polymerase chain reaction; random amplified polymorphic DNA
Subjects: Food systems > Food security, food quality and human health
Crop husbandry > Production systems > Fruit and berries > Olive
Values, standards and certification
Research affiliation: Italy > CRA - National Council of Agricultural Research
Deposited By: Perri, Dr. Enzo
ID Code:10236
Deposited On:27 Dec 2006
Last Modified:12 Apr 2010 07:34
Document Language:English
Status:Published
Refereed:Peer-reviewed and accepted

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