10236: RAPD-PCR amplification of DNA from virgin olive oil
(2002) RAPD-PCR amplification of DNA from virgin olive oil. Paper presented at 4th International Symposium on Olive Growing, Valenzano, Italy, 25-30 September, 2000; Published in Vitagliano, C. and Martelli, G. P., Eds. Acta Horticulturae 586, page pp. 583-586.
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Summary
The characterization of the genetic identity of DNA recoverable from olive oil could facilitate the identification of the source cultivar. This requires that intact DNA or large fragments suitable for amplification by polymerase chain reaction (PCR) should be recovered from olive oil. This paper presents the preliminary results of an experiment evaluating the extent to which DNA recoverable from the veiled virgin olive (cv. Carolea) oil would allow PCR amplification useful for random amplified polymorphic DNA (RAPD) fingerprinting. Only residual DNA from olive oil sediments obtained by protease treatment was useful in RAPD-PCR amplification.
| Document Language: | English |
|---|---|
| Keywords: | DNA; olive oil; olives; polymerase chain reaction; random amplified polymorphic DNA |
| Subject Areas: | Food systems > Food security, food quality and human health Crop husbandry > Production systems > Fruit and berries > Olive Values, standards and certification |
| Research affiliation: | Italy > CRA |
| Total budget (Euro): | 0 |
| Orgprints ID Number: | 10236 |
| Contact: | Perri, Dr. Enzo |
| Deposited On: | 27 December 2006 |
| EPrint Type: | Conference paper |
| Published?: | Published |
| Online at: | http://search.epnet.com/login.aspx?direct=true&db=lah&an=20033049294 |
| Peer Review Status: | Peer-reviewed and accepted |
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