Organic Eprints frontpage
 about    browse    search    register    user area    help 

10236: RAPD-PCR amplification of DNA from virgin olive oil

Perri, Dr Enzo; Muzzalupo, PhD Innocenzo and Sirianni, Ms Raffaella (2002) RAPD-PCR amplification of DNA from virgin olive oil. Paper presented at 4th International Symposium on Olive Growing, Valenzano, Italy, 25-30 September, 2000; Published in Vitagliano, C. and Martelli, G. P., Eds. Acta Horticulturae 586, page pp. 583-586.

Full text available as:
Other (MS Word)

Summary

The characterization of the genetic identity of DNA recoverable from olive oil could facilitate the identification of the source cultivar. This requires that intact DNA or large fragments suitable for amplification by polymerase chain reaction (PCR) should be recovered from olive oil. This paper presents the preliminary results of an experiment evaluating the extent to which DNA recoverable from the veiled virgin olive (cv. Carolea) oil would allow PCR amplification useful for random amplified polymorphic DNA (RAPD) fingerprinting. Only residual DNA from olive oil sediments obtained by protease treatment was useful in RAPD-PCR amplification.

Document Language:English
Keywords:DNA; olive oil; olives; polymerase chain reaction; random amplified polymorphic DNA
Subject Areas: Food systems > Food security, food quality and human health
Crop husbandry > Production systems > Fruit and berries > Olive
Values, standards and certification
Research affiliation: Italy > CRA
Total budget (Euro):0
Orgprints ID Number:10236
Contact:Perri, Dr. Enzo
Deposited On:27 December 2006
EPrint Type:Conference paper
Published?:Published
Online at:http://search.epnet.com/login.aspx?direct=true&db=lah&an=20033049294
Peer Review Status:Peer-reviewed and accepted

Archive Staff Only: edit this record